Table 2.
Analysis of WT and (R58A)10WT1 TRAP binding to (GAGAA)11 RNA by stopped-flow fluorescence
| RNA | WT TRAP ka × 106 M−1 sec−1 | (R58A)10WT1 ka × 106 M−1 sec−1 |
|---|---|---|
| GAGAU-1a | 100.4 ± 3.7b | 1.69 ± 0.04 |
| GAGAU-2 | 109.2 ± 3.7 | - |
| GAGAU-3 | 119.3 ± 3.5 | - |
| GAGAU-4 | 60.8 ± 1.0 | - |
| GAGAU-5 | 56.7 ± 3.1 | - |
| GAGAU-6 | 45.9 ± 1.7 | - |
| GAGAU-7 | 50.7 ± 2.8 | - |
| GAGAU-8 | 51.8 ± 0.9 | - |
| GAGAU-9 | 44.1 ± 1.8 | - |
| GAGAU-10 | 38.1 ± 4.1 | 1.3 ± 0.05 |
| PolyA-GAGAU-1 | 20.4 ± 2.0 |
a
RNA consisted of 10 GAGAA repeats and one GAGAU repeat in which the U residue is replaced with Fluorescein-12-U. The location of the GAGAU repeat is indicated by the number; for example in GAGAU-1 the single U is contained in the spacer region after the first GAG repeat.
b
Association rate constants are the average of at least 3 individual experiments using at least 5 different concentrations of TRAP.