Table II.
Yeast two-hybrid interactions of μ-chains with sorting signals
| Protein | Signal | Growth on TDO | Growth on TDO + 1 mM 3-AT | Growth on QDO |
|---|---|---|---|---|
| LDLR (proximal) | NPVYQKT | — | — | — |
| LDLR (distal) | QDGYSYPSR | μ2, μ3A, and μ4 | — | — |
| TfnR (proximal) | PLSYTRF | μ1A, μ1B, μ2, μ3A, and μ4 | μ2 and μ4 | μ4 |
| TfnR (distal) | VDGNDSHV | — | — | — |
| TGN38 | ASDYQRL | μ1A, μ1B, μ2, μ3A, and μ4 | μ2, μ3A, and μ4 | μ3A and μ4 |
| VSVG | RQIYTDI | μ1B, μ1Bmut, and μ4 | μ1B and μ1Bmut | — |
Data were obtained as described in Materials and methods, and images of yeast plates showing growth of cotransformants on selective media are shown in Fig. S4 (available at http://www.jcb.org/cgi/content/full/jcb.200610047/DC1). TDO, triple dropout; QDO, quadruple dropout. 3-AT, 3-amino-1,2,4-triazole.