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. 2007 Aug 3;7:148. doi: 10.1186/1471-2407-7-148

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Copyright © 2007 Morrissey et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A: The tartrate-resistant acid phosphatase assay was performed according to the manufacturer's instructions on mouse serum samples from control, prevention, and treatment groups, using the Mouse TRAP™ Assay measuring TRACP-5b activity (Immunodiagnostic systems Inc.) (n = 10). There were no significant differences associated with huRANKL MAb administration. B: Serum calcium levels were measured using a calcium ion electrode 8 weeks after intra-tibial C4-2 injection in the control, prevention, and treatment groups. Serum calcium levels were significantly different between control and prevention animals (p = 0.012). Serum calcium levels were not significantly different between control and treatment animals (p = 0.9631).