Figure 2.

Stability of cytokine phenotype upon immediate restimulation. The cell populations primed twice in the experiment described in Fig. 1 were also immediately restimulated in vitro with peptide, APC, IL-2, and anti-IL-4 and IL-12 or IL-4. Seven days later, the cells were challenged with peptide, APC, and IL-2, and cytosolic cytokine content was determined by flow cytometry. Cytokine secretion was also measured. 4, IL-4; γ, IFN-γ.