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. 1997 Apr 1;94(7):3189–3194. doi: 10.1073/pnas.94.7.3189

Figure 4.

Figure 4

Requirements for TH2 cells primed in the absence of IFN-γ to develop IFN-γ-producing activity. TG CD4+ T cells were primed with peptide, APC, IL-2, IL-4, anti-IL-12, and anti-IFN-γ. The cells were then restimulated with peptide, APC, IL-2, anti-IL-12 and IL-4 or anti-IL-4 and IFN-γ or anti-IFN-γ. At the end of the restimulation culture, they were challenged with peptide, APC, and IL-12 or anti-IL-12. Cytosolic cytokine content was measured. As a control, cells that had been primed and restimulated in the presence of anti-IL-4 and IL-12 were also tested.