Table 1. Summary of the effects of heterogenous nucleating agents.
| Protein | Control (no nucleant) | Fumed silica | CM Sephadex | Sand | Titanium(IV) oxide | Glass wool | Hydroxy-apatite | Cellulose | Horse hair | Dried seaweed | Combination of 9 nucleants | Combination of 4 nucleants | |||||||||||
| Total | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | + | − | |
| Lysozyme | 5 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 4 | 0 | 7 | 3 | 7 | 0 |
| Pepsin | 3 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 4 | 1 | ND | |
| Trypsin | 5 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 3 | 0 | 5 | 1 | ND | |
| Glucose Isomerase | 4 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 0 | 3 | 1 | ND | |
| Ribonuclease A | 6 | 0 | 4 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 3 | 1 | 2 | 1 | 3 | 2 | ND | |
| Myoglobin | 4 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 4 | 0 | ND | |
| α-lactalbumin | 5 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | ND | |
| Catalase | 5 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 3 | 1 | 1 | 0 | 6 | 2 | ND | |
| Xylanase | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 3 | 1 | 2 | 1 | 6 | 1 | ND | |
| Thaumatin | 4 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 | 0 | 2 | 1 | 5 | 1 | ND | |
| Sum over all proteins | 46 | 8 | 22 | 3 | 12 | 0 | 1 | 5 | 6 | 1 | 0 | 17 | 10 | 16 | 3 | 20 | 4 | 23 | 3 | 43 | 12 | ND | |
| Total effect (crystallization hits) | −14 | −9 | −1 | −1 | 1 | 7 | 13 | 16 | 20 | 31 | ND | ||||||||||||
| Total effect (%) | −30 | −20 | −2 | −2 | 2 | 15 | 28 | 35 | 43 | 67 | ND | ||||||||||||
For each protein/heterogeneous nucleating agent pair and the sum over all proteins, the number of new and missing crystallization conditions (“+” and “−”, respectively) is shown, relative to the no-nucleant control. For the no-nucleant control, the total number of crystallization hits is shown. The total effect of the nucleating agent, relative to the no-nucleant control, is shown in the last two rows. “ND”, not determined.