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. Author manuscript; available in PMC: 2007 Oct 18.

Published in final edited form as: Immunity. 2007 Aug;27(2):281–295. doi: 10.1016/j.immuni.2007.07.010

T-bet functions in both MPECs and SLECs according to an expression gradient.

(A) Naïve and day 8 KLRG1^hi IL-7R^lo SLECs or KLRG1^lo IL-7R^hi MPECs were sorted and examined by Western blotting T-bet and GRp94 levels.

(B) wt and T-bet^−/− P14 CD8 T cells were analyzed 5, 8 and 30 days pi for CD122 expression.

(C) T-bet^+/+, T-bet^+/− or T-bet^−/− P14 CD8 T cells were analyzed 7 and 30 days pi for KLRG1 and IL-7R expression and T-bet expression (bottom histogram plot).

(D) T-bet^+/+, T-bet^+/− or T-bet^−/− P14 memory CD8 T cells were analyzed 30 days pi for CD122 expression

(E) T-bet^−/− P14 CD8 T cells were transduced with T-bet RV or one expressing T-bet fused to the estrogen receptor (T-bet:ER) and transferred into mice subsequently infected with LCMV. Mice were treated with 0 – 8 mg of Tamoxifen (Tm) during infection and on day 7 pi, GFP⁺ donor splenocytes were analyzed for expression of KLRG1 and IL-7R.