Table 2.
Expression of CBF-A increases the efficiency of translation in HEK293 cells of mRNA that contains 62 or 99 (CGG) repeats
| Expressed protein | None | CBF-A | CBF-A ΔRNP11 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| (CGG) repeats | FL mRNA | FL protein | FL protein/ mRNA | FL mRNA | FL protein | FL protein/ mRNA | FL mRNA | FL protein | FL protein/ mRNA |
| 0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| 30 | 1.9 ± 0.6 (9) | 2.5 ± 1.2 (10) | 1.3 | 2.1 ± 0.3 (5) | 5.0 ± 1.2 (5) | 2.3 | 1.6 ± 0.4 (5) | 3.3 ± 0.8 (3) | 2.1 |
| 62 | 1.1 ± 0.3 (8) | 1.4 ± 0.7 (7) | 1.3 | 1.8 ± 0.3 (4) | 3.6 ± 0.4 (3) | 2.0 | 1.6 ± 0.3 (4) | 3.1 ± 0.1 (2) | 1.9 |
| 99 | 5.9 ± 1.7 (6) | 3.6 ± 1.5 (6) | 0.6 | 7.2 ± 1.7 (4) | 13.7 ± 3.7 (4) | 1.9 | 6.4 ± 1.9 (5) | 4.4 ± 0.5 (3) | 0.7 |
The calculated average ratios of FL protein to mRNA are in bold.