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Schematic diagrams for the use of Oxa as a linker to directly immobilize probe DNA oligomers on the NH2-functionalized surface. Through the activation-free reactivity of Oxa-nucleotide unit (Oxa-N) with amino groups, the probe DNA oligomers with Oxa-N (Oxa-Probe, Oxa-c3-Probe and Oxa-c12-Probe) are covalently attached on the surface in one-pot reaction.
