Table 1.
Probe and target DNA oligomers used in this study
| Probe DNA oligomers | |
|---|---|
| Oxa-Probe | 5′-d(OTGTTGTCGAAAATGTCAACG)-3′ |
| Oxa-c3-Probe | 5′-d(Oc3TGTTGTCGAAAATGTCAACG)-3′ |
| Oxa-c12-Probe | 5′-d(Oc12TGTTGTCGAAAATGTCAACG)-3′ |
| Oxa-Probe-F | 5′-d(OTGTTGTCGAAAATGTCAACGF)-3′ |
| Oxa-c3-Probe-F | 5′-d(Oc3TGTTGTCGAAAATGTCAACGF)-3′ |
| Oxa-c12-Probe-F | 5′-d(Oc12TGTTGTCGAAAATGTCAACG F)-3′ |
| Target DNA oligomers | |
| Cy3-XDHt-S | 5′-d(Cy3 CGTTGACATTTTCGACAACA)-3′ |
| Cy3-XDHt-L | 5′-d(Cy3 CTGCTGCTGTCGCCAAGACCT |
| TCGGTGCTAAGGGTGTCATCGT | |
| CGTTGACATTTTCGACAACAAG | |
| TTGAAGATGGCCAAGGACATT | |
| GGTGCTGCTACTCACACCTT)-3′ | |
| Non-complementary | 5′-d(Cy3 GCAACTGTAAAAGCTGTTGT)-3′ |
O: Oxanine, F: Fluorescein, c3: Carbon 3 spacer (–(CH2)3–), c12: Carbon 12 spacer (–(CH2)12–), Cy3: Cy3 fluorescence label; In Cy3-XDHt-L, underlined bases are the same sequence as Cy3-XDHt-S.