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. 2007 Aug 24;35(17):5861–5873. doi: 10.1093/nar/gkm637

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Restriction digest analysis of IntDOT recombinant. (A) The recombination reaction mediated by IntDOT occurs in the presence of E. coli IHF. The reaction occurs between a linear radiolabeled (asterisk) attB DNA and an attDOT site cloned on a plasmid. The product of the reaction is a linear 3.6 kb long recombinant. Cleavage by SspI should generate fragments of 1.1 and 2.5 kb. (B) Agarose gel analysis of recombination products. Lane 1, 3.6 kb long linear recombinant. Lane 2, 1 kb ladder. Lane 3, 1 kb ladder. Lane 4, products of the recombination reaction digested with SspI enzyme. Lane 5, the recombination reaction with no IntDOT and IHF added.