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EMSA experiments between a 146 bp double-stranded oligonucleotide and (A) Mm53BP1[1463–1617], (B) ScRad9[754–947]. Increasing concentrations of proteins were incubated with a 146 bp DNA fragment at a concentration of 26 nM: 2-fold (lanes 2 and 7), 8-fold (lanes 3 and 8), 32-fold (lanes 4 and 9) and 128-fold (lanes 5 and 10) molar excess of proteins were used. Lanes 1 and 6 indicate the mobility of naked DNA.
