Table 1.

Affinity constants (µM) of ScRad9[754–947] and ScRad9[754–931] for different oligonucleotides. Experiments with ScRad9[754–947] were carried out with and without 1 mM DTT to measure the influence of cysteine oxidation on DNA binding. However, similar results were found in both conditions. Interactions for which the affinity was not determined are displayed as ‘nd’.

Kd (µM)	357 bp ds	211 bp ds	146 bp ds	35 bp ds	35 bp Nick	35 bp mismatch GT	35 bp Gap	35 bp ss	35 bp ds with a 3′ terminal biotin	35 bp with a 3′ terminal biotin + 0.5 µg/µl streptavidin
ScRad9[754–947] with DTT	0.42 ± 0.06	0.50 ± 0.07	0.34 ± 0.07	0.60 ± 0.14	nd	nd	nd	2.74 ± 0.27	0.83 ± 0.05	1.39 ± 0.08
ScRad9[754–947] without DTT	nd	nd	nd	0.96 ± 0.08	0.62 ± 0.06	0.89 ± 0.07	0.96 ± 0.13	2.50 ± 0.18	nd	nd
ScRad9[754–931] with DTT	nd	nd	nd	2.07 ± 0.27	nd	nd	nd	5.02 ± 0.67	nd	nd