Figure 3.

Common fold, different properties and distinct functions for XPF and ERCC1. Sequence conservation either for XPF or ERCC1 proteins is colored from white (non-conserved) to red (highly conserved). The opposite face has no significant conservation for either protein. Hydrophobic side chains are colored orange. Electrostatic surface potentials were calculated using APBS (39) and colored blue for positive or red for negative charge potential. Active site residues of XPF were chosen based on previous mutational studies (5) and the XPA-binding site of ERCC1 was identified by our NMR titrations.