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. 2007 Aug 22;35(17):5717–5728. doi: 10.1093/nar/gkm588

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The percentage of the total observed base changes corresponding to each different transition and all transversions (grouped) following the single- or multi-cycle SPEX amplification of various ancient extracts. SC = Single-cycle SPEX. MC = Multi-cycle SPEX. Cons = CDS. Total = TCDS. TrV = transversions. SC-Bison = 6 ancient bison extracts: (Cons) 7,654 bp, 337 discrete sequences; (Total) 10,644 bp, 548 independent amplicons. MC-Bison = 5 ancient bison extracts: (Cons) 4,187 bp, 171 discrete sequences; (Total) 4,934 bp, 219 independent amplicons. MC-Human = 4 ancient human extracts (at 3 loci): (Cons) 3304 nucleotides, 164 discrete sequences; (Total) 4,647 bp, 271 independent amplicons. MC-Cavelion = 3 ancient Eurasian cave lion extracts: (Cons) 4,086 bp, 111 discrete sequences; (Total) 4,470 bp, 141 independent amplicons.