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. 2007 Aug 24;35(17):5809–5818. doi: 10.1093/nar/gkm613

Figure 5.

Figure 5.

Pif1p binding to forked and unforked substrates. Increasing amounts of Pif1p were incubated at 25°C with 50 pM nucleic acid substrate, in absence (a) or presence (b) of the nonhydrolyzable nucleotide analog AMPPNP, and analyzed on 12% non-denaturing gels as described in the Materials and Methods section. Data from three different experiments were averaged and plotted. Error bars represent the SD between the three experiments. Binding parameters are as follows; in the absence of a nucleotide, Kd(D20) = 1.0 × 10−7 M; Kd(R20) = 1.7 × 10−8 M; Kd(fD20) = 1.3 × 10−7 M; Kd(fR20) = 5.2 × 10−8 M; in the presence of AMPPNP, Kd(D20) = 2.0 × 10−6 M; Kd(R20) = 3.2 × 10−6 M; Kd(fD20) = 3.2 × 10−7 M; Kd(fR20) = 1.4 × 10−6 M.