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. 2007 Aug 28;35(17):5922–5933. doi: 10.1093/nar/gkm632

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — I-PpoI expression and cleavage of genomic rDNA repeats in Sua 4.0 cell lines. (a) Schematic map of A. gambiae rDNA clusters and the location of the I-PpoI site within the 28S rDNA. ITS, internally transcribed spacer; Hc, HincII (HindII). (b) Southern blot analysis of A. gambiae Sua 4.0 cells transfected with pEGFP-Ppo or pEGFP-Ppo H98A. Genomic DNA was digested with ClaI and a 2 kb fragment of rDNA amplified with primers rDfwd and rDrev was used as probe. DNA in lanes marked by a (+) was digested with I-PpoI in vitro. (c) Primer extension analysis. Genomic DNA extracted from cells transfected with pEGFP-Ppo or pEGFP-Ppo H98A was digested with HincII and extended with primer rPrex. DNA in lanes marked by a (+) was digested with I-PpoI in vitro.