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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Anal Biochem. 2007 Mar 18;366(1):59–70. doi: 10.1016/j.ab.2007.03.012

Figure 4.

Figure 4

Glyceryl lipids containing palmitic acid (16:0) as revealed by the neutral loss scan for 273.3u during electrospray ionization of neutral lipids from control RAW 264.7. The identity of abundant [M+NH4]+ ions are indicated by the abbreviations for each fatty acyl group present in the diacylglycerol ions and the total number of fatty acyl carbon atoms and total number of fatty acyl double bonds present in triacylglycerol molecular species. The glyceryl lipids were isolated from RAW 264.7 cells (approximately 5 × 106 cells) after treatment for 1 hr with Kdo2-lipid A to which a stock solution of D5-TAG and D5-DAG internal standards (600 pmol each internal standard) was added prior to extraction.