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Detection of a hypersensitive site at −308. In vitro DNase I footprint analysis of TNF1 allele (coding strand) is shown. The unfilled arrowhead indicates an area of protection, and the filled arrowhead a DNase I hypersensitive site. Lanes: 1, Maxam–Gilbert guanidine ladder; 2, naked DNA control; 3, plus Raji cell nuclear extract.
