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. Author manuscript; available in PMC: 2008 May 18.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Mar 19;356(4):906–911. doi: 10.1016/j.bbrc.2007.03.061

FIG. 2. Binding of Annexin II with SHP2 was affected by extra cellular calcium chelator.

FIG. 2

Western blot analysis was carried out with lysates of the COS-1 cells pre-treated with the indicated calcium chelators for 20 min, and then either untreated or treated with EGF (100 ng/ml) for 10 min. SHP2, Annexin II or Src antibodies were used in western blots to detect the levels of the specific proteins (Input). The cell lysates were immunoprecipitated with anti-SHP2 polyclonal antibodies, followed by western blot analysis using anti -SHP2, -Annexin II or -4G10 antibodies. As a control, cell lysates were immunoprecipitated with normal rabbit serum, first lanes in lower panel.