Figure 7.

Myosin sequence alignments

Sequence alignment of various myosins showing exon 7 (in the boxed area), together with loop 1, switch I and the relay area. “*” means that the residues are identical in all sequences in the alignment.“:” represents conserved substitutions, whereas “.” indicates semi-conserved substitutions. Only two residues in exon 7 are 100 % conserved (G³¹⁸ and D³²⁷) of which D³²⁷ forms a salt-bridge towards the highly conserved R²⁸³ (scallop numbering). Note that myosin VI has an insert in the middle of the exon 7 domain. The corresponding cardiomyopathy sites in the exon 7 domain and helix L are indicated with a ▼.