Figure 1.
Transduction susceptibility mediated by BacCMV-GFP in mammalian cells. 105 cells were transduced at a multiplicity of infection (MOI) of 100 with BacCMV-GFP. (a) Drawing showing the cassette structure of BacCMV-GFP. Abbreviations: CMV, cytomegalovirus immediately-early promoter/enhancer (nucleotides -655 to +106); EGFP, enhanced green-fluorescent protein; BGHpA, bovine growth hormone poly adenylation sequence. (b) Representative histograms obtained by flow cytometry after 48 h post-transduction. The percentage of GFP+ cells is reported in the insets, and was calculated by subtracting the background obtained with mock transduced cells (see Methods). Numbers in the inset refer to the percentage of GFP+ cells ± SD without (above) or with (below) treatment with butyrate. (c) Percentage of GFP+ cells determined by FACS analysis in either the presence or absence of butyrate. *P < 0.01, **P < 0.05, ***P = 0.7244 versus cells non-treated with butyrate. NS = non significant. d) Levels of expression of GFP and induction ration (indicated on the right) as determined by the mean fluorescence intensity in the presence or absence of butyrate. Values are means ± SD of four independent experiments.