Figure 3.

Inhibition of the in vivo priming of LN cells to the myasthenogenic peptides by the dual analog given 7 days following immunization. (A) SJL and (B) BALB/c mice were injected intradermally in the hindfoot pads with 10 μg of p195-212 and 20 μg of p259-271, respectively, in CFA. Analog Lys-262–Ala-207 (200 μg in 500 μl of PBS) was injected i.p. (SJL mice, ○) and i.v. (BALB/c mice, □) 7 days after the priming. LN cells obtained from the mice were incubated in the presence of various concentrations of p195-212 or p259-271 for 96 hr. Thereafter, [³H]thymidine was added and 16 hr later cells were harvested and radioactivity was counted. Results are expressed as mean cpm of triplicate cultures. SD values did not exceed 10%.