Table 1.
Binding of peptides to MHC class II on APCs
| Biotinylated peptide
|
||||
|---|---|---|---|---|
| Mouse strain | p195–212 | p259–271 | Ala-207–Lys-262 | Lys-262–Ala-202 |
| SJL | 0.3 × 10−6 M | No binding | 5 × 10−6 M | 0.5 × 10−6 M |
| BALB/c | Not done | 3.4 × 10−6 M | 8 × 10−6 M | 3.9 × 10−6 M |
APCs (106 cells per sample) of SJL and BALB/c mice were incubated for 20 hr in the presence and absence of each of the biotinylated peptides: p195-212, p259-271, 207-Ala–Lys-262, and Lys-262–Ala-207. Phycoerythrin–streptavidin staining was followed as described. Thereafter, cells were subjected to flow cytometric analysis. Double-reciprocal conversion of the net mean fluorescence intensity (MFI) values (after subtraction of background fluorescence) was used to calculate the Kd-like constants.