Figure 1.—

Mutation of the zim genes suppresses the Cog and Egl defects of egl-13(ku207). The histogram illustrates the Cog⁺ and Egl⁺ percentage of egl-13(ku207) and suppressor; egl-13(ku207) double mutants. The original zim-3(tm2303) chromosome was associated with linked mutations causing lethality (Phillips and Dernburg 2006). Thus, we used an unc-24(e138) zim-3(tm2303) recombinant chromosome, which causes less lethality (Phillips and Dernburg 2006), to assay suppression by zim-3 and tested unc-24(e138) as a control. Cog and Egl phenotypes were scored as reported (Hanna-Rose and Han 1999). The him-8(e1489) data were published previously (Nelms and Hanna-Rose 2006) and shown here for comparison. Note that wild-type animals have 100% normal gonad morphology and egg-laying ability. For Figures 1–3, basic principles of proportional analysis were applied as previously described to obtain 95% confidence intervals shown as error bars (Nelms and Hanna-Rose 2006). Actual percentage is cited within each bar, and sample sizes (n) are indicated at the right in Figures 1–3. Asterisks indicate values for double mutants in Figures 1–3 that are significantly different from the relevant single mutants (P < 0.005, Fisher's exact test).