Figure 4.—

him-8(e1489) increases the intensity of lin-48∷GFP expression in the hindgut of an egl-38(gu22) mutant. (A) Fluorescent image of the hindgut region of an saIs14[lin-48∷GFP] hermaphrodite overlaid on the corresponding DIC image. (B) lin-48∷GFP expression is visible in four hindgut cells that flank the rectum. (C) Expression is consistently detected in all four cells at similar intensities in the transgenic parent strain. (D) The intensity of lin-48∷GFP expression in him-8(e1489); saIs14[lin-48∷GFP] animals is not detectably different from that of the parent strain. (E) Thirty-one percent of egl-38(gu22); unc-119(e2498); saIs14[lin-48∷GFP] animals lack all hindgut expression of lin-48∷GFP (Figure 3), and the intensity of lin-48∷GFP expression is weaker than that of the parental strain in all animals that have expression. (F) In addition to an increase in the percentage of the population that expresses the reporter faithfully (Figure 3), all suppressed egl-38(gu22) him-8(e1489); saIs14[lin-48∷GFP] animals have an increase in the intensity of lin-48∷GFP expression relative to the parental strain. Image exposure conditions were identical for A and C–F.