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petite accumulation in strain D273-CD3-Δmip1 transformed with different MIP1 alleles
| % petites
|
||
|---|---|---|
| D273-CD3-Δmip1 | 28° | 36° |
| MIP1[S] | 5.1 ± 0.4 | 22.6 ± 3.4 |
| MIP1[S]A661T | 2.2 ± 0.2 | 2.6 ± 0.3 |
D273-CD3-Δmip1 is a spontaneous ura3 mutant of D273-10B/A1 in which the MIP1 gene has been replaced by a KanR deletion cassette in the presence of the pFL38 (Bonneaud et al. 1991) plasmid-borne MIP1[S] or MIP1[S]A661T to keep mtDNA. Experimental conditions are as in Figure 1A.
