TABLE 1.

Summary of DNA diversity in S. latifolia and S. dioica chloroplast and autosomal genes

			All sites						Nonsilenta				Silentb
Gene	Taxon	Nc	Length	Sd	π(%)e	θ (%)f	Dg	HKAh	Length	Sd	π(%)e	θ (%)f	Length	Sd	π(%)e	θ (%)f
Chloroplast
TrnTLF	Elisanthe	92	1644	31	0.165	0.37	−1.71	NS	55	0	0	0	1589	31	0.171	0.383
	S. latifolia	65	1645	19	0.136	0.243	−1.34	NS	55	0	0	0	1590	19	0.141	0.252
	S. dioica	23	1655	17	0.186	0.278	−1.07	NS	55	0	0	0	1600	17	0.118	0.288
MatK	Elisanthe	101	1014	27	0.18	0.51	−1.95*	P = 0.0029	788.7	15	0.115	0.367	225.3	12	0.411	1.03
	S. latifolia	69	1014	18	0.14	0.37	−1.86*	P = 0.0008	788.72	11	0.077	0.29	225.28	7	0.362	0.647
	S. dioica	27	1014	14	0.22	0.36	−1.29	P = 0.0007	788.65	6	0.186	0.197	225.35	8	0.354	0.921
RbcL	Elisanthe	69	1314	6	0.02	0.1	−1.80*	NS	992.02	1	0.006	0.021	321.98	5	0.078	0.323
	S. latifolia	50	1314	2	0.02	0.03	−1.01	NS	992.01	1	0.008	0.023	321.99	1	0.036	0.069
	S. dioica	15	1314	3	0.04	0.07	−1.32	NS	992.04	0	0	0	321.96	3	0.16	0.287
TrnTLF	Elisanthe	87	2658	53	0.171	0.396	−1.87*	P = 0.033	843.7	13	0.114	0.327	1799.6	40	0.202	0.441
+ MatK	S. latifolia	62	2659	35	0.141	0.28	−1.63*	P = 0.0135	843.73	9	0.077	0.243	1800.6	26	0.174	0.307
	S. dioica	22	2669	29	0.202	0.298	−1.24	P = 0.0061	843.64	6	0.199	0.209	1809.7	23	0.211	0.349
Autosomal
Slop	Elisanthe	70	2198	146	0.696	1.43	−1.76		166	3	0.05	0.27	2028	148	0.75	1.51
	S. latifolia	42	2221	93	0.607	1.01	−1.44		166.02	2	0.06	0.2	2055	94	0.65	1.06
	S. dioica	28	2312	75	0.554	0.845	−1.39		165.97	1	0.03	0.11	2146	75	0.59	0.91

DNA diversity statistics and neutrality tests were conducted in the programs: ProSeq (Filatov 2002) and DnaSP (Rozas et al. 2003).

^aFor the trnL gene encoding tRNA for leucine, all positions in exons are counted as non-silent.

^bSilent sites: synonymous and noncoding.

^cSample size.

^dNumber of polymorphic sites.

^eAverage heterozygosity per 100 sites (Nei 1987, equation 10.5).

^fWatterson's estimator of scaled mutation rate (2N_eμ) per 100 sites (Watterson 1975).

^gTajima's D (Tajima 1989), significance or rejection of neutrality is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

^hComparison of the amount of polymorphism in the chloroplast genes and the autosomal Slop gene by the HKA test of neutrality (Hudson et al. 1987).