Figure 2.—

Many of the genes that suppress the his3-nonstop phenotype also increase mRNA levels of a second nonstop mRNA reporter. Strains isolated as suppressors of his3-nonstop were transformed with a plasmid containing a Protein A-nonstop reporter. The steady-state level of Protein A-nonstop mRNA was assayed by Northern blot. Shown is the average and the spread of two independent experiments after correcting for loading difference using a probe for the RNA subunit of the signal recognition particle (7S RNA). The mRNA level is expressed as a value relative to that found in an isogenic wild-type strain.