Figure 6.—

Mutants that increase steady-state levels of the Protein A-nonstop mRNA also increase stability of the mRNA. Each of the indicated strains was transformed with a plasmid directing expression of the Protein A-nonstop reporter from a GAL1 promoter. mRNA stability was assayed by inhibiting transcription from the GAL1 promoter with the addition of glucose and isolating RNA at the indicated time points. The mRNA remaining at various points after transcription inhibition was quantitated. Plotted is the average mRNA remaining from three independent experiments for each mutant and from four independent experiments for the wild type.