TABLE 2.
Genes that suppress the nonstop phenotype
| Protein A-nonstop
|
|||||
|---|---|---|---|---|---|
| Gene | Function/putative function | his3-nonstop | Steady state | Stability | dcp synthetic lethal |
| SKI2 | Exosome-mediated mRNA degradation | ++ | ND | ND | ND |
| SKI3 | Exosome-mediated mRNA degradation | ++ | 2.0 | 2.7 | + |
| SKI7 | Exosome-mediated mRNA degradation | ++ | 2.7 | 2.5 | + |
| SKI8 | Exosome-mediated mRNA degradation | ++ | 2.1 | 2.5 | + |
| PRE9 | Proteasome-mediated protein degradation | ++ | 0.4 | ND | ND |
| UMP1 | Proteasome-mediated protein degradation | ++ | 0.4 | ND | ND |
| yMR247c | Proteasome-mediated protein degradation | +++ | 0.6 | ND | ND |
| SSN2 | Subunit of the RNA polymerase II mediator; interacts with mRNA deadenylase | ++ | 2.6 | 1.1 | − |
| SSN3 | Kinase of the RNA polymerase II mediator; interacts with mRNA deadenylase | ++ | 2.3 | 1.5 | − |
| MED1 | Subunit of the RNA polymerase II mediator | ++ | 1.5 | 1.2 | − |
| IPK1 | mRNA export, RNA editing, inositol phosphate kinase | ++ | 3.0 | 1.6 | − |
| HTZ1 | Variant of histone H2 | ++ | 2.0 | 1.1 | + |
| SIR3 | Gene silencing | ++ | 1.7 | 1.4 | ND |
| yGR122w | Unknown | ++ | 1.5 | ND | ND |
| NUP2 | Subunit of the nuclear pore complex | ++ | 2.1 | 0.9 | + |
| yLR021w | Unknown | ++ | 0.5 | 1.7 | + |
| Wild type | — | − | 1.0 | 0.9 | − |
His3-nonstop growth is indicated as −, indicating little or no growth; ++, indicating significant growth; and +++, indicating growth that was even more pronounced. dcp2-7 synthetic lethality is indicated with a + sign and − sign indicates no synthetic growth defect. ND, not determined.