TABLE 2.
The phenotypes of fli-1(bp130) mutant animals carrying various transgenes
| Genotype | Brood size | No. of germ cellsa | EMOb | No. of seam cells |
|---|---|---|---|---|
| Wild type | 189.0 ± 32.2 (n = 5) | 320.4 ± 42.5 (n = 5) | 0 (n = 5) | 16.3 ± 0.4 (n = 32) |
| fli-1(bp130) | 13.0 ± 8.6 (n = 8) | 78.4 ± 34.2 (n = 12) | 6 (n = 8)c | 11.2 ± 0.5 (n = 83) |
| fli-1(bp130); fli-1∷gfp | ||||
| Line 1 | 32.0 ± 8.4 (n = 2) | 189.3 ± 10.1 (n = 3) | 1 (n = 10) | 14.5 ± 1.3 (n = 4) |
| Line 2 | 38.5 ± 4.9 (n = 4) | 192.5 ± 30.1 (n = 4) | 1 (n = 9) | 15.3 ± 0.75 (n = 6) |
| Line 3 | 68.3 ± 7.4 (n = 3) | 210.0 ± 62.3 (n = 4) | 0 (n = 12) | 15.7 ± 1.0 (n = 6) |
| Line 4 | 58.7 ± 6.3 (n = 3) | 86.5 ± 32.4 (n = 2) | 2 (n = 11) | 15.6 ± 1.3 (n = 8) |
| fli-1(bp130); scm∷fli-1∷gfp | ||||
| Line 1 | 18.2 ± 5.8 (n = 5) | 76.3 ± 8.9 (n = 3) | 8 (n = 8) | 14.7 ± 1.6 (n = 6) |
| Line 2 | 11.6 ± 6.0 (n = 3) | 89.2 ± 12.6 (n = 5) | 8 (n = 9) | 16.2 ± 0.6 (n = 7) |
| Line 3 | 12.6 ± 2.3 (n = 3) | 92.5 ± 10.6 (n = 2) | 7 (n = 7) | 13.8 ± 1.2 (n = 4) |
| fli-1(bp130); Fli I∷gfp | ||||
| Line 1 | 64.3 ± 10.6 (n = 3) | 168.3 ± 34.2 (n = 3) | 1 (n = 8) | 15.7 ± 2.3 (n = 7) |
| Line 2 | 74.5 ± 13.8 (n = 4) | 154.5 ± 20.2 (n = 4) | 0 (n = 9) | 16.2 ± 1.2 (n = 6) |
| Line 3 | 56.0 ± 5.7 (n = 2) | 192.0 ± 27.7 (n = 4) | 1 (n = 12) | 14.3 ± 1.5 (n = 9) |
a
The number of germ cells was counted in each gonad arm by DAPI staining.
b
The number of animals that displayed the EMO phenotype.
c
The other two mutants were sterile. The total number of animals examined are shown in parentheses.