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. 2007 Sep 20;26(20):4368–4379. doi: 10.1038/sj.emboj.7601845

Figure 3.

Figure 3

SiRNA-mediated knockdown of OGT impairs activation of NFAT and NFκB upon TCR stimulation. (A) Jurkat cells stably expressing a luciferase under the control of the NFAT promoter were transfected with either a negative control siRNA (Ctrl) or siRNAs specific for Lck or OGT (OGT_1, OGT_2, OGT_3). Cells were incubated for 24, 48 or 72 h and then costimulated with plate-bound anti-CD3/28 antibodies for 5.5 h. luciferase activity was quantified as described in Materials and methods. Experiments were performed in triplicates. (B) Jurkat cells stably expressing a luciferase under the control of the NFκB promoter were transfected with a negative Ctrl or siRNAs specific for Lck or OGT (OGT_1, OGT_2, OGT_3). Cells were treated as described in (A).