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. 2007 Sep 20;26(20):4335–4346. doi: 10.1038/sj.emboj.7601861

Figure 1.

Figure 1

coa1Δ cells are respiratory deficient and have low mitochondrial copper. (A) Copper levels were assessed by ICP-OES in Nycodenz-purified mitochondria of coa1Δ cells and the isogenic WT BY4743 grown in YPD. (B) coa1Δ cells and the WT BY4743 were grown in YPD liquid media at 30°C with 1 mM CuSO4, where indicated and oxygen consumption (% O2/sec/OD600) was measured. (C) BY4743 and coa1Δ cells were grown in YPD then serially diluted and spotted on YP 2% glycerol–2% lactate (G/L) or 2% glucose plates. CuSO4, FeCl2 and ZnSO4 were added to the plate media at specified concentrations. Plates were kept at 30°C for 5 days.