Figure 11.

Model for Coa1 linking Cox1 translation to cofactors assembly. After the translation of Cox1 mRNA, which depends on Pet309 and Mss51, Mss51 interacts with Cox1 and associates with Cox14 (Barrientos et al, 2004). This ternary complex is recruited by Coa1 and likely forms the ∼400-kDa complex that we detected by BN-PAGE. Membrane insertion of Cox1 probably occurs via Oxa1. Shy1 joins the complex and promotes the dissociation of Cox14 and Mss51 that is recycled to its translational function. The cofactor insertion in Cox1 can then proceed via Cox10, Cox15 (heme a biosynthesis) and Cox11 (Cu_B insertion) leading to holo-Cox1.