Figure 4.
High-copy suppressors of the respiratory defect of coa1Δ cells. (A) coa1Δ cells transformed with high-copy vectors isolated from the suppressor screen were grown in SC-2% raffinose, serially diluted and spotted on SC 2% glycerol–2% lactate or 2% glucose (control) with selection. The plates were incubated at 30°C for 2 days (glucose) or 7 days (glycerol-lactate). (B) The same cells as in panel A were spotted on rich media (YP) supplemented with 50 μM FeCl2, containing 2% glycerol–2% lactate (YPLG) or 2% glucose (YPD), and either the copper-specific chelator BCS or CuSO4. The plates were incubated at 30°C for 2 days (YPD) or 6 days (YPLG).