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. 2007 Sep 20;26(20):4335–4346. doi: 10.1038/sj.emboj.7601861

Figure 5.

Figure 5

Restoration of CcO by high-copy MSS51 in coa1Δ cells. (A) coa1Δ cells with high-copy MSS51 or an empty vector (vec) were grown in SC-2% raffinose and assayed for oxygen consumption. (B) Mitochondria were purified from these cells and assayed for CcO activity. (C) Immunoblot of mitochondria from coa1Δ cells containing an empty vector (vec), a low-copy vector expressing COA1 or a high-copy vector expressing MSS51. (D) Heme profile from mitochondria purified from coa1Δ containing high-copy MSS51 or an empty vector. A/B represents the ratio of the area under the curve for the peaks of heme b (B) and heme a (A). (E) coa1Δ cells expressing high-copy MSS51 and containing an empty vector (vec) or high-copy vectors with MDJ1 or COX10 were grown in SC-2% raffinose, serially diluted and spotted on SC 2% glycerol–2% lactate or 2% glucose with selection. The plates were incubated at 30°C for 2 days (glucose) or 7 days (glycerol-lactate).