Fig. 3. Regulation of ER activity by HDI is a general phenomenon.
A. HeLa cells were transfected with ERE2-TK-LUC reporter construct along with CMV-5 (●) CMV-hERα (■) or CMV-hERβ (△) expression vectors and treated with ethanol control vehicle (C) or increasing concentrations of E2, without or with TSA (500 ng/ml). Results are expressed as percent of values obtained in the absence of E2. B and C. Same conditions as in A to analyze the effects of E2 (10−8M), propyl pyrazole triol (PPT, 10−8M), diarylpropionitrile (DPN, 10−8M), hydroxy-tamoxifen (OHTam, 10−8M) or ICI 182,780 (ICI, 10−8M).