Figure 2.

Treatment of rat plasma with peroxynitrite. Concentration-dependent formation of protein-bound NTyr. Rat plasma (200 μl) was treated with peroxynitrite (•) and processed according to Materials and Methods. Rat plasma was also treated with peroxynitrite that was allowed to decompose at pH 7.2 for 15 min before addition (○). Values represent the average of three determinations ± SD. ∗, P < 0.05; ∗∗, P < 0.01, one-tailed Students t test.