Table 3.

Formation of protein-bound NTyr by zymosan and IFN-γ-treated RAW 264.7 macrophages

	AcATyr per 106 AcTyr	Nitrite, μM
Untreated	1.05 ± 0.46	4.6 ± 1.0
Zymosan	5.13 ± 0.57*	53.5 ± 1.2
IFN-γ	1.30 ± 0.49	11.1 ± 2.9
Zymosan + IFN-γ	7.52 ± 0.13*†	71.0 ± 3.7
Zymosan + IFN-γ + NMMA	1.30 ± 0.43	12.4 ± 0.4

Values are expressed as mean ± SD (n = 4 determinations for all conditions). 

^*P < 0.01 vs. untreated control;  

^†P < 0.001 (zymosan + IFN-γ vs. Zymosan); P values calculated by one-way ANOVA, Bonferroni multiple comparison test.