Figure 4.
Expression and function of the recombinant hAQP1 in vivo. (A) Ten micrograms of crude membranes from rat submandibular glands, which were cannulated and infected for 24 h with 5 × 109 pfu of either Addl312 or AdhAQP1, were used for Western blot analysis as in Fig. 1. (B) Submandibular glands of sham-irradiated (Non-IR) or irradiated (IR) rats were cannulated and retrograde instilled with 5 × 109 pfu of either Addl312 (hashed bar) or AdhAQP1 (solid bar), and then saliva was collected after 3 days as described in Methods. The results are expressed as saliva secretion in microliters per 100 g of rat body weight per 15 min and are the mean ± SEM. The results were analyzed using an ANOVA. ∗, P < 0.05 between the two groups with different instilled viruses.