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Western blot. Lane 1, synthetic cecropin A, 5 μl of a 1.0 mM aqueous solution (4.1 kDa). Lanes 2–6, hind gut preparations from five third-instar nymphs of R. prolixus (R1–R5) carrying R. rhodnii, which has been transformed to express the cecropin A gene product. Bioassays (Fig. 4) showed that all five specimens (R1–R5) had significant biological activity attributable to cecropin A, though only R4 and R5 had sufficient cecropin A to be detected in our Western blot system.
