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Fas mRNA induction is deficient in Pkro/o cells. Pkr+/+ or Pkro/o MEFs were stimulated with the indicated inducers for 6 h. Fas mRNA levels were detected by reverse transcription–PCR (35 cycles) and analysed using 2% agarose gel electrophoresis. Detection of glyceraldehyde-3-phosphate dehyrdogenase (GAPDH) mRNA by reverse transcription–PCR (30 cycles) was used as a control for general RNA integrity and equivalent loading.
