Figure 1.

Polyacrylamide gel electrophoresis of purified recombinant LT, TNF, and Mut2. Each sample (3 mg) was suspended in sample buffer containing 5% 2-mercaptoethanol, 3% SDS, and 65 mM Tris·HCl (pH 6.8) and heated at 100°C for 5 min. The samples were subjected to SDS/PAGE (8 × 10 × 0.1 cm) in 15% gels for 2 hr at 150 V and stained with Coomassie brilliant blue R-250.