Table 2.
Antitumor effect of Mut2 on various mouse tumors in comparison with TNF or LT
| Tumor | Mut2
|
TNF
|
LT
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| ED50 | LD50 | TI | ED50 | LD50 | TI | ED50 | LD50 | TI | |
| Colon 26 | 0.3 | 6.9 | 23.0 | 0.3 | 1.5 | 5.0 | 0.3 | 2.3 | 7.7 |
| Sarcoma 180 | 0.2 | 1.2 | 6.0 | 0.2 | >4.0* | >20.0 | 0.3 | 1.2 | 4.0 |
| B16 | 7.3 | >12.0* | >1.6 | >1.3† | 1.2 | <0.9 | 11.9 | >12.0* | >1.0 |
| (ED16) | |||||||||
| M5076 | 0.8 | >12.0* | >15.0 | 0.9 | >4.0† | >4.4 | 3.0 | >12.0* | >4.0 |
| (LD20) | |||||||||
| Colon 38 | 1.4 | >12.0* | >8.6 | >3.0† | >3.0* | >1.0 | 6.6 | >12.0* | >1.8 |
| (ED9) | |||||||||
| Meth A | 0.32 | 7.5 | 23.4 | 0.3 | 1.1 | 3.7 | 0.62 | 4.0 | 6.5 |
Cytokines were given twice a week for a total of five injections. ED50 and LD50 values were expressed as milligrams per kilogram, and represent the dosages given at each injection.
No toxic death was observed with the dose indicated.
ED50 or LD50 was not determined. ED values represent the injection dose that gave maximum inhibition. For example, ED16 means the value that gave 16% inhibition of the tumor growth. Values noted beside LD indicate the percentage of animals that died with the dose administered. For example, LD20 indicates the dose that killed 20% of the animals. On day 1 tumor cells were implanted, and cytokine treatment was started on day 9 for Colon 26, on day 4 for Sarcoma 180, on day 11 for B16, and on day 10 for M5076 and Colon 38. The ED50 value was calculated by using relative tumor weight on day 14 for Colon 26, on day 20 for Sarcoma 180, on day 14 for B16 and M5076, and on day 25 for Colon 38, after the initial cytokine administration.