Figure 5.

Dose-dependent sulindac sulfide inhibition of PGE₂ production and cell invasion. Either control or COX-2-expressing Caco-2 cells were treated with sulindac sulfide at the indicated concentrations for 24 hr prior to harvest. In A, PGE₂ concentrations were determined and the results plotted on the y axis. The cells were then evaluated in the invasion chamber assay described. The number of cells that invaded through the Matrigel and lodged onto the filter were counted and the results plotted on the y axis in B. Results are expressed as number of cells per high power field (×400). Five separate fields were counted per filter and the experiment was repeated four separate times.