Figure 1.
(A) Unique RNAs are found in receptor-expressing TCMK-1 cells. Total RNA from TCMK-1 or mouse L cells was probed with a 32P-labeled fragment of RTMCR-4 to reveal differentially expressed 6-kb (large arrow) and 1.4-kb (small arrow) RNAs. (B) Amino acid alignment of HCAR and MCAR. The deduced amino acid sequences of the cloned HCAR and MCAR cDNAs were aligned using the GeneWorks software program. Vertical lines, amino acid identities; dashes, gaps in the alignment. The positions of the potential signal peptide (thin underline), transmembrane region (thick underline), and N-linked glycosylation sites (∗) are shown. (C) A domain model for the CAR proteins. IG1 and IG2, immunoglobulin domains; SS, signal sequence; TM, transmembrane-spanning region.