Figure 2.

(A) pRTHR and pRTMR confer susceptibility to Ad5 entry. Only (A) TCMK-1 positive controls or NIH 3T3 cells transfected with either (B) pRTHR or (C) pRTMR stained for β-galactosidase expression after incubation with a CMV-β-galactosidase recombinant Ad5 vector at a multiplicity of infection of around 50. (D) pBK-CMV controls showed no reactivity after incubation under identical conditions. (B) HCAR expression is localized to the exterior of the cell. Bright staining was observed over the entire plasma membrane in unfixed NIH 3T3 cells transfected with pRTHR and labeling transfectants with RmcB and a fluorescein isothiocyanate-labeled goat anti-mouse antibody (A). B represents pBK-CMV control cells.