Figure 4.

HCAR and MCAR mRNA is expressed in a variety of tissues. (A) Hybridizing mRNAs of 6 kb and 1.4 kb can be detected in poly(A)⁺ Northern blots of TCMK-1 cells, but not mouse L cells. In contrast, six species of mRNAs are detected in HeLa cells that range in size from 6 kb to 1.2 kb that are not found in receptor-negative rhabdomyosarcoma (Rd) cells. The sizes of the RNAs that correlate with the size of the isolated HCAR (large arrow) and MCAR (small arrow) cDNAs are shown. (B) Human multiple tissue Northern blots were purchased from CLONTECH and hybridized with a ³²P-labeled fragment that corresponded to the open reading frame of HCAR. Hybridizing sequences are similar in size to those detected in HeLa cells. (C) Mouse multiple tissue Northern blots (CLONTECH) were hybridized with a ³²P-labeled fragment that corresponded to the open reading frame of MCAR. Hybridizing sequences are similar in size to those detected in TCMK-1 cells.