Table 1.

Production of plaque-forming units after virus infection of transfected NIH 3T3 cells

Plasmid*	Virus yield, plaque-forming units†
	CVB3	CVB4	Poliovirus
pRTHR	2.92  ×  107	1.7  ×  106	2.65  ×  104
pRTMR	4.22  ×  106	6.0  ×  105	2.60  ×  104
pBK-CMV	2.25  ×  104	1.5  ×  103	2.27  ×  104

^*Plasmids encoding HCAR (pRTHR), MCAR (pRTMR), and vector alone (pBK-CMV) were transfected into receptor-negative NIH 3T3 cells and assayed for virus production 24 h post-transfection with a multiplicity of infection of around 50. 

^†Virus titers of transfected cell supernatants were determined by plaque assay on HeLa cells 24 h postinfection as described (8).